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KMID : 0811720030070000191
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Korean Journal of Physiology & Pharmacology
2003 Volume.7 No. 0 p.191 ~ p.0
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Simulation of Osmotic Pressure Split Experiment: Implication of Intracellular Water Flow Resistance
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Leem Chae-Hun
Noma Akinori
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Abstract
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It has been suggested that cytosolic environment was different from the free solution. The cytosolic viscosity and diffusion coefficient has been measured but intracellular water flow resistance has not been studied. In 1999, there was report that, when the osmotically different solutions were applied to each half of the cells, the width change of each half were opposite (Sasaki, 1999). We tried to simulate this experiment. We assumed that the net ionic flux through membrane were zero but water can pass through membrane. Intracellular water was moved by osmotic pressure difference. The intracellular ion such as K, Na, and Cl can be moved by diffusion and water convection. We could succssfully simulate the experimental results. Based on Poisseulle’s principles, we found the water resistance in cytosol was very larger up to 1.5×10 (8) than that in free solution. The water flow resistance through membrane was 4.0×10 (11). From Poisseulle’s principles, the viscosity must increase the same amount as resistance but it can not be true. We need to apply Darcy’s principle which describe the water movement through porous medium. From this pricinples, the Darcy’s permeability are 2.98×10 (-11) um2 in membrane and 5.3×10 (-9) um2 in cytosol, when water viscosity was 0.1 cP. From this results, cytosolic water is in a gel status.
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KEYWORD
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Water flow resistance, Darcy’s permeability, Gel, Osmotic pressure split
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